Does pregnyl lose potency after 60 days?

Technically yes, but realistically no.

Most Pregnyl will come listed with a "60 day discard" date, as pharmacies are required to have 100% sterility & potency by that date per regulations.

After that date, it does not necessarily mean it is expired or inert, but that is what a pharmaceutical manufacturer will list on the vial for their own personal liability.

The date on the vial does not directly translate to the strength of the medication or how effective it will be. It is solely how long sterility is guaranteed.

After that date, the benzoyl alcohol in it begins to degrade and it becomes less effective at killing bacteria in the solution. Technically, the medication should remain as effective so long as it remains refrigerated. Even if someone leaves a peptide hormone unrefrigerated, it still only loses about 7% efficacy after 60 days.

So although the discard dates do have factual merit, they more exist due to liability guarantees on the behalf of the producer than what might make the most sense for use.

Since HCG typically comes in 10,000 units commercially, having a lower use dose of 500 units weekly should mean that 10,000 units lasts 20 weeks. It would be very expensive for men to use HCG & have to replace it every 60 days given how much it costs when commercially acquired. For lower doses & longer use cases, it is just as potent as long as it remains refrigerated