is there a big difference in injecting ,same weekly dose, once a week vs twice a week vs three times a week vs EOD vs ED. I have tried all and don't see a big difference but I did notice my E2 was higher on ED and EOD injections

Generally we don't have enough data to compare between the two, as you'd really need a man who has done both & testing multiple times with both at the same dosing and habits. Otherwise each man is so variable with his exact absorption that drawing conclusions probably isn't wise.

That said, it is absolutely true that you will have a more consistent level with more frequent dosing. In practice though, very few men would need to be so intently dialed in. The rare cases would be where someone absolutely does not want to take an AI but would like to get as high as possible as consistently as possible without going over a threshold that causes a lot of Estrogen transfer for them. This isn't really something that happens, though, as most men can handle the normal fluctuations or can slightly adjust their dose. We do sometimes swap to three times weekly over two times weekly, but there are diminishing returns to going EoD or ED after already doing that.

You'd probably not be able to notice a difference between twice weekly & other forms given what you've shared, meaning you're a prime example of someone who small fluctuations don't bother.